2020 Volume 69 Issue 3 Pages 360-365
[Introduction] A reagent for measuring lipase using the synthetic substrate 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6-methyl-resorufin)-ester (DGGMR), which reacts specifically with lipase, has been developed. However, a high triglyceride (TG) concentration in serum was reported to cause a false negative error for lipase measured by “Cygnus Auto LIP.” Therefore, the purpose of this study was to clarify the effect of serum TG concentration on lipase concentration. [Method] We investigated the correlation between lipase concentration measured by Cygnus Auto LIP or Liquitech Lipase Color II and serum TG concentration. We studied the effect of each lipoprotein fraction on the change rate of lipase concentration. [Results] A high serum TG concentration caused a false negative error for lipase, and there was a significant negative correlation between them (r = −0.867, p ≤ 0.001). When serum TG concentrations exceeded 400 mg/dL, the false negative error for lipase reached 19%. A lipoprotein fraction analysis showed that VLDL had the strongest effect on the false negative error for lipase. [Discussion] Competition inhibition caused by lipoprotein particle size was considered to be a factor for the false negative error for lipase. Although it may have little impact on daily clinical practice, we need to pay attention to this error in serum samples with high TG concentrations, especially high VLDL or high chylomicron concentration.
