2021 Volume 70 Issue 1 Pages 93-98
(1→3)β-D glucan (BG) is widely used as a biomarker for auxiliary serological diagnosis of deep mycosis. The number of measurements per day is limited because conventional BG reagents are processed by batch. We evaluated the utility of “Fungitec G test ES “NISSUI”” (NISSUI BG), which is a real-time processing reagent based on the colorimetric method. The within-run precision and the dilution linearity were satisfactory. In 116 patients, the measurement value of NISSUI BG was approximately three times higher than that of the “β-glucan test Wako (Wako BG)”, which is based on the turbidimetric method. We evaluated the concordance rates of the judgment using the cutoff values of each reagent. The results showed that eight samples are mismatched, such that all “NISSUI BG” results were positive. However, some of the results were suspected as having false high values, and three of the samples indicated the presence of fungi. It was suggested that “NISSUI BG” sensitively reflected the presence of fungi in the body. Therefore, when the result of “NISSUI BG” was positive, it was suggested that a follow-up by measuring BG and performing culture tests and confirming both the clinical symptoms and the background of patients presenting false high values were needed. It was considered that “NISSUI BG” was useful for routine laboratory tests because of the high reactivity for BG and the short time to obtain results owing to the real-time processing.
