Japanese Journal of Medical Technology
Online ISSN : 2188-5346
Print ISSN : 0915-8669
ISSN-L : 0915-8669
Technical Articles
Evaluation of IFCC-based reagent kit L-type Wako ALP·IFCC and reactivity of samples under on-board state
Manabu OKUBOSatoko HURUKAWAChihiro KIMURAHitomi MAEDASatoe UESUGIYutaka KOHGUCHIKaoru TOHYAMA
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2021 Volume 70 Issue 2 Pages 273-278

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Abstract

We evaluated the IFCC-based reagent kit L-type Wako ALP·IFCC and the reactivity of samples under the on-board state. The within-run precision obtained was 0.6%. The dilution linearity and the lower limit of detection were 990 and 5.4 U/L, respectively. The reagent stability in the analysis laboratory and the serum (liver and intestine) ALP activities decreased by 7.9–8.2% after 30 days, but QAP troll IX and IIX were stable (< 3.2%). The correlation coefficient (r) between the IFCC-based reagent and the JSCC-based reagent was 0.997, and the ALP activities determined using the IFCC-based reagent were 37% of the ALP activities determined using the JSCC-based reagent. The basal performance of the IFCC-based reagent is satisfactory and it could be used for measurement with high sensitivity.

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© 2021 Japanese Association of Medical Technologists
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