2021 Volume 70 Issue 2 Pages 252-259
We study the adhesion between a specimen and a glass slide and try to prevent the detachment of intra-operatively obtained frozen HE specimens of the mammary margin. First, we used tissue paraffin sections and the remaining cytology material to study specimen adhesion to a coated glass slide. As a result, we found that to achieve adhesion of specimens that can withstand staining, the paraffin tissue sections must be wetted with water, and both paraffin tissue sections and cytology specimens required adequate water diffusion through drying and dehydration from the adhesion surface to a coated glass slide. Next, we examined the prevention of detachment of intra-operatively obtained frozen HE specimens of the mammary margin, and found that, compared with wet fixation, dry fixation significantly prevented specimen detachment (p < 0.001). Therefore, the choice of the fixation method for specimen preparation was important. In practice, after spray fixation, which is one of the cytological specimen fixation methods, specimen detachment is prevented by drying the specimen, and it was possible to prepare intra-operatively obtained frozen HE specimens of the mammary margin with good staining.
