2021 Volume 70 Issue 3 Pages 482-488
In this study, we evaluated the performance of a new method of measuring Presepsin, the “HISCL Presepsin”. Presepsin was measured with the HISCL Presepsin reagent using HISCL-5000. The control reagent was STACIA CLEIA Presepsin. Existing samples were measured. We evaluated whether HISCL Presepsin is satisfactory in terms of precision, long-term stability, linearity, detection limit, interference from substances, correlations, and method of mixing. Satisfactory results in terms of precision and linearity were obtained．There was good long-term stability for nine weeks．The detection limit when using the 2SD method was 9.4 pg/mL. There was no interference from substances such as hemoglobin, unconjugated bilirubin, conjugated bilirubin, and rheumatoid factor．A strong correlation was observed between the HISCL Presepsin and STACIA CLEIA Presepsin (r = 0.997, y = 0.91x − 89.21). In addition, we examined three effects of agitation on the measurement of Presepsin. The STACIA CLEIA Presepsin showed a larger increase in percentage of rate of increase test value. However, we need to pay attention to the handling of premeasurement samples because of the possibility of an abnormally high Presepsin concentration caused by excessive agitation. In summary, our results suggest that HISCL Presepsin may be useful for the early diagnosis of sepsis.
