Japanese Journal of Medical Technology
Online ISSN : 2188-5346
Print ISSN : 0915-8669
ISSN-L : 0915-8669
Technical Articles
Evaluation of five media for detection of Staphylococcus aureus and seven media for detection of methicillin-resistant S. aureus
Hiromi TOChiemi TOKUSHIGEFumiyoshi ERANaoko HIGUCHIMakiko YUKINozomi KAJIHARAChinami OBAHirofumi SHIMADA
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2021 Volume 70 Issue 4 Pages 685-690

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Abstract

To efficiently isolate methicillin-resistant Staphylococcus aureus (MRSA) using culture media, we evaluated the performance of five media for the detection of Staphylococcus aureus, and we compared the performance of seven media for the detection of MRSA with that of the drug susceptibility test. Sixty Staphylococcus strains (28 MRSA strains, 17 methicillin-susceptible Staphylococcus aureus (MSSA) strains, and 15 non-S. aureus strains) were used for evaluation of culture media. As a result, the sensitivity and specificity of culture media for the detection of S. aureus were 91%–100% and 87%–100%, respectively. Moreover, in comparison with the drug susceptibility test, the positive and negative predictive values for MRSA were 90%–100% and 94%–100%, respectively. We recommend that the properties of media used to efficiently isolate MRSA should be clarified in future studies.

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© 2021 Japanese Association of Medical Technologists
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