Reexamination of morphological diameter of peripheral blood cells and effect of drying method on diameter

Abstract

To evaluate cell diameter values in the literature, smears of peripheral blood from eight (21 to 23 years old) subjects were prepared by the wedge method. May-Grünwald-Giemsa staining was then performed and cell diameters were measured from digital images. Measurement results were then compared with literature values for red blood cells, platelets, and the five white blood cell fractions of the subjects. In addition, the effects of two drying methods, forced drying and natural drying, on cell diameters were compared. The diameters measured from digital images of various peripheral blood cells in this study differed depending on red blood cells, platelets, and the five white blood cells described in the literature. No significant differences in cell diameters were observed between forced drying and natural drying.