2025 Volume 74 Issue 2 Pages 340-346
Latex agglutination immunoturbidimetry is an immunological assay for pulmonary surfactant protein D (SP-D), but assay devices and reagents are limited. In this study, we conducted a basic performance evaluation of the reagent “Nanopia SP-D”, which can now be measured by a general-purpose automated analyzer. We evaluated the accuracy, analytical range, specificity, sample storage stability, and comparison with a control method, and obtained good results for all items. In terms of specimen storage stability, an increase in measured values was observed in specimens stored at room temperature from the 1st day to the 10th day, but the detailed cause of this increase was not clear. In comparison with the control method, some high value samples showed a bias in measured values, and some samples showed deviations in measured values from the control method, suggesting the possibility of storage conditions of samples or accidental errors. The high sensitivity of the test reagent is expected to contribute to early detection and prompt therapeutic intervention in lung-related disorders such as alveolar proteinosis and interstitial pneumonia.
