石油の増回収を目的とした炭化水素利用菌の集積培養に関する研究(第4報)

2F-NW 8901菌株の利用に関する研究

抄録

Our previous studies^1-3) showed a bacterium 2F-NW 8901 among several ten bacteria could be useful for MEOR. In the present study, conditions for enrichment culture of 2F-NW 8901 in an oil bed were investigated.
Results may be summarized as follows.
1. Supplying oxygen by any manner of means is required for active growth, but supply of sugar is not required.
2. Since 2F-NW 8901 grows relatively fast and lives at the anaerobic condition, a short-time supply or cyclic supply of air may be sufficient for growth.
3. Surface active agents produced may be only the metabolites in hydrocabon oxidation pathways.
4. Adding the yeast extract or equivalents to the liquid medium is effective for MEOR.
5. Because the concentration of Fe²⁺ ions in a formation water is generally less than that required for the growth of bacteria, adding Fe²⁺ may be effective for the growth of 2F-NW 8901.
6. 2F-NW 8901 is Al³⁺ tolerant to some extent, so that the enrichment culture may be effectively done with the presence of low concentration Al³⁺ ions.