Abstract
The ultimate purpose of basic and clinical research in tissue regeneration is to recover its function, esthetics, and psychological factor. It is believed that the combination of fig 1 is needed depending on its size, location, or target cell. For example, in severe defect pattern all the factors above are needed. Therefore the key for clinical success is to select the most effective protocol in each therapy. Recently, in maxillofacial surgical field, there are three concepts; physical barrier (such as space making or barrier membrane technique), activation of autogenous cell (such as cortification or autogenous graft), and growth factor (such as in PRP, BMP, Emdogain). Those concepts are utilized singularly or utilized as in combination of two or three. Moreover, safety and simplicity is important to apply the concepts in clinical medicine. In this paper, we discuss an easy and effective method to produce and store PRP before the day of the actual operation. We investigated the use of frozen PRP on the activity of platelets. We also investigated the use of collagen gel as a substitute for thrombin, in order to avoid any chance of infection from human produced thrombin. The possibility of harvesting osteogenic mesenchymal cells from the jaw was also investigated.
