2012 年 10 巻 2 号 p. 59-66
In the present study, three-dimensional (3D) porous poly(lactic acid) (PLA) scaffold was fabricated by using progen and freeze-dry method and then fibronectin was immobilized after NaOH treatment on 3D porous scaffold. The biological effect of fibronectin immobilization on PLA scaffold was evaluated by the measurement of initial attachment of MC3T3-E1 osteoblast-like cells and by the morphological observation of attached cells. The number of attached cells onto FN-PLA scaffolds were significantly greater than on PLA scaffolds after 90 min culture (p<0.05). The attached cells were flattened and fastened on FN-PLA scaffold surface and the presence of lamelipodia and flopodia was recognized. Moreover the attached cell could be observed not only the surface of 3D porous PLA scaffold but also the inside of the scaffold by fibronectin immobilization. On the other hand, cells were rounded shape on PLA scaffold surface and there are no formation of lamelipodia and flopodia. The attached cells were not seen in the inside area of PLA scaffold.In conclusion, it revealed that fibronectin immobilization of 3D porous PLA scaffold was effective for biological activities of osteoblast-like cells.