Abstract
Porcine atelocollagen has traditionally been used as a scaffold for cell culture in the field of regenerative medicine. Marine collagen, rather than collagen derived from mammals has recently attracted attention. However, the collagen of fish (e.g., salmon) that inhabits cold oceans has already been demonstrated to be unsuitable as a three-dimensional scaffold for long-term culture at 37°C. In the present study, we examined whether type I collagen, extracted from the scales of tilapia inhabiting tropical and subtropical areas, can be used as a scaffold for ES cells. As a result, it had no effect on ES cell differentiation as compared with conventional porcine collagen. In a previous study, salmon collagen could not withstand long-term culture. Thus, the collagen obtained from tilapia, featuring a high denaturation temperature (35-37°C), is useful as a scaffold for ES cell differentiation.
