The Effect of Artificial Recombinant Bone Morphogenetic Protein 2 with Titanium-binding Peptide on a Ti Graft in Vivo

Eitoyo KOKUBU; Katsutoshi KOKUBUN; Kenichi MATSUZAKA; Kenji KASHIWAGI; Kiyotaka SHIBA; Takashi INOUE; Kazuyuki ISHIHARA; Masao YOSHINARI

doi:10.11223/jarde.12.1

ORIGINAL ARTICLES

The Effect of Artificial Recombinant Bone Morphogenetic Protein 2 with Titanium-binding Peptide on a Ti Graft in Vivo

Eitoyo KOKUBU, Katsutoshi KOKUBUN, Kenichi MATSUZAKA, Kenji KASHIWAGI, Kiyotaka SHIBA, Takashi INOUE, Kazuyuki ISHIHARA, Masao YOSHINARI

Author information

Eitoyo KOKUBU
Oral Health Science Center, and Department of Microbiology, Tokyo Dental College, Tokyo, Japan
Katsutoshi KOKUBUN
Oral Health Science Center, and Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan
Kenichi MATSUZAKA
Oral Health Science Center, and Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan
Kenji KASHIWAGI
Division of Protein Engineering. The Cancer Institute of the Japanese foundation for Cancer Research, Tokyo, Japan
Kiyotaka SHIBA
Division of Protein Engineering. The Cancer Institute of the Japanese foundation for Cancer Research, Tokyo, Japan
Takashi INOUE
Oral Health Science Center, and Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan
Kazuyuki ISHIHARA
Oral Health Science Center, and Department of Microbiology, Tokyo Dental College, Tokyo, Japan
Masao YOSHINARI
Oral Health Science Center, and Department of Dental Materials Science, Tokyo Dental College, Tokyo, Japan

Keywords: titanium-binding peptide, bone morphogenetic protein-2, artificial protein

JOURNAL FREE ACCESS

2014 Volume 12 Issue 1 Pages 1-7

DOI https://doi.org/10.11223/jarde.12.1

Details

Abstract

Surface modification of titanium (Ti) influences cell behavior, and immobilization of bone morphogenetic protein (BMP)-2 on a Ti surface cells to differentiate into osteogenic cells. This study aimed to evaluate the effect of an artificial fusion protein (AFP) comprising a modified BMP-2 and Ti-binding peptide (TBP) motif (AFP-TBP–BMP-2) on osteogenesis of rat abdominal cells on Ti surface in vivo. Ti plates were dipped in three different mixtures of collagen gel: collagen gel only, collagen gel with BMP-2, and collagen gel with AFP-TBP–BMP-2. The treated Ti plates were then implanted into rat abdominal muscles. One and 2 weeks after implantation, these plates were histologically observed. The Ti plate with collagen gel and AFP-TBP–BMP-2 produced cartilage in the muscle at 1 week, and bone-like hard tissue was observed at 2 weeks. These results suggest that the application of collagen gel with AFP-TBP–BMP-2 accelerates osteogenesis in vivo.

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