2014 年 12 巻 1 号 p. 1-7
Surface modification of titanium (Ti) influences cell behavior, and immobilization of bone morphogenetic protein (BMP)-2 on a Ti surface cells to differentiate into osteogenic cells. This study aimed to evaluate the effect of an artificial fusion protein (AFP) comprising a modified BMP-2 and Ti-binding peptide (TBP) motif (AFP-TBP–BMP-2) on osteogenesis of rat abdominal cells on Ti surface in vivo. Ti plates were dipped in three different mixtures of collagen gel: collagen gel only, collagen gel with BMP-2, and collagen gel with AFP-TBP–BMP-2. The treated Ti plates were then implanted into rat abdominal muscles. One and 2 weeks after implantation, these plates were histologically observed. The Ti plate with collagen gel and AFP-TBP–BMP-2 produced cartilage in the muscle at 1 week, and bone-like hard tissue was observed at 2 weeks. These results suggest that the application of collagen gel with AFP-TBP–BMP-2 accelerates osteogenesis in vivo.