Journal of Oral Tissue Engineering
Online ISSN : 1880-0823
Print ISSN : 1348-9623
ISSN-L : 1348-9623
Study of Hybrid Culture Technique using Human Hepatocytes and Mouse ES Cells by Three-dimensional Culture Scaffolds
Koichi IMAITsubasa SHIRAIYoshitomo HONDATetsunari NISHIKAWATomoharu OKAMURAAkio TANAKAFumiya OGAWAHirofumi SAWAIMuneyasu SHIDAKazuhiko SUESE
ジャーナル フリー

2015 年 12 巻 3 号 p. 121-126


An in vitro embryonic stem cell test allows the screening of potential reproductive and developmental toxicities, but does not reflect human metabolic activators. Thus, a novel test for the hybrid cultures of hepatocytes and mouse ES cells should be developed. To develop procedures for the hybrid cultures of three kinds of hepatocyte and mouse ES cells, basic structures for hybrid cultures and two scaffolds for three-dimensional cultures were examined to determine optimal experimental conditions.In the comparison of basic structures for hybrid cultures, the incidence was slightly higher with hepatocytes placed outside the inter cell and embryo bodies (EBs) inside. As a result of the comparison of the scaffolds, glass fiber showed a slightly higher incidence than collagen gel scaffolds.

© 2015 by Japanese Association of Regenerative Dentistry
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