Protective Effect of Molecular Hydrogen on Antimycin A-induced Injury in H9c2 Cardiomyocytes

Abstract

Periodontitis is common in adults and cardiovascular diseases are the most common cause of adult death in the world. However opening of the infarct-related coronary artery is a valued therapeutic goal in acute myocardial infarction, cardiomyocytes continue to die during reperfusion. Acute oxidative stress induced by ischemia –reperfusion causes serious damage to tissue. The purpose of our study was to investigate that hydrogen (H₂) gas has potential as an antioxidant in preventive and therapeutic applications. We first determined the dose at which cytotoxicity develops in a period of 1 h upon antimycin A (AA) exposure in H9c2 cells. AA (0-500 μM) exhibited cytotoxicity in a concentration-dependent manner. A maximum reduction was 76.5 ± 4 of the control group (at 500 μM of AA). AA-induced cytotoxicity in H9c2 cells were improved by H₂ gas. These results suggested the protective effects H₂ gas against AA- induced injury in H9c2 cells.