Preparation and Biodegradation Properties of DNA/poly-lysine Complexes by FE-SEM

抄録

We prepared DNA/cationic polylysine complexes, and examined their biodegradation behavior and histopathological responses for use as a scaffold material for bone regeneration. For the cationic polylysine, two types of isomeric polylysine, D-polylysine (DNA/D-lysine complex) and L-polylysine (DNA/L-lysine complex) were used. A water-insoluble white powder of the complexes with a porous structure was obtained. The yields of the DNA/D-lysine complexes and DNA/L-lysine complexes were 63.8% and 64.5%, respectively. There were mild tissue responses after a disk comprising either complex was implanted into the subcutaneous tissue of rats. The biodegradation rate of the DNA/L-lysine complexes was greater than that of the DNA/D-lysine complexes. This study suggested that DNA/cationic polylysine complexes have the potential for controlled biodegradation using satisfactory combinations of isomeric polylysine.