Influence of Sr²⁺ on Strontium Substituted Hydroxyapatite's(Sr-HA) Cytotoxicity

抄録

The synthetic pure hydroxyapatite(HA) have excellent biocompatibility and a little osseous inductivity, however, there are still some shortcomings such as the high degree of crystallibility and the quite stability of the structure. Strontium seemed to have the potential to change the biocompatibility and physicochemical characteristics of HA for implant use. Sr-HA containing 1%, 5%, 10%, 100% Sr²⁺ were synthesized by solution-reaction system. MTT assay and Flow Cytometry (FCM) method were used to test the cytotoxicity of Sr-HA bioceramic. Result shows that there's no obvious cytotoxicity of all the Sr-HA bioceramics. But the cytotoxicity increases as the Strontium concentration raising. As the FCM method shows, there's no apparent difference between the pure hydroxyapatite and all of the Sr-HA. Through this in vitro study on cytotoxicity we can come to the conclusion that the Sr-HA bioceramics have no obvious cytotoxicity and may have good biocompatibility, especially 1%, 5% Sr-HA.