Effects of Low-intensity Pulsed Ultrasound on Bone Formation with                    Gelatin Hydrogel Containing Bone Morphogenetic Protein-2

Kozo YAMAJI; Akihito YOKOYAMA; Tsutomu SUGAYA; Kumiko MATSUZAKI; Kei TAKAHASHI; Yasuo SHINNO; Naoko OHARA; Shigeki SUZUKI

doi:10.11223/jarde.22.53

ORIGINAL ARTICLES

Effects of Low-intensity Pulsed Ultrasound on Bone Formation with Gelatin Hydrogel Containing Bone Morphogenetic Protein-2

Kozo YAMAJI , Akihito YOKOYAMA, Tsutomu SUGAYA, Kumiko MATSUZAKI, Kei TAKAHASHI, Yasuo SHINNO, Naoko OHARA, Shigeki SUZUKI

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Kozo YAMAJI Corresponding author
Department of Operative Dentistry, Faculty of Medicine, Dentistry and Pharmaceutical Science, Okayama University, Okayama, Japan
Akihito YOKOYAMA
Department of Operative Dentistry, Faculty of Medicine, Dentistry and Pharmaceutical Science, Okayama University, Okayama, Japan
Tsutomu SUGAYA
Department of Periodontology and Endodontology, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan
Kumiko MATSUZAKI
Division of Hospital Dentistry, Central Clinical Department, Okayama University Hospital, Okayama, Japan
Kei TAKAHASHI
Department of Operative Dentistry, Faculty of Medicine, Dentistry and Pharmaceutical Science, Okayama University, Okayama, Japan
Yasuo SHINNO
Department of Operative Dentistry, Faculty of Medicine, Dentistry and Pharmaceutical Science, Okayama University, Okayama, Japan
Naoko OHARA
Department of Operative Dentistry, Faculty of Medicine, Dentistry and Pharmaceutical Science, Okayama University, Okayama, Japan
Shigeki SUZUKI
Department of Operative Dentistry, Faculty of Medicine, Dentistry and Pharmaceutical Science, Okayama University, Okayama, Japan

Keywords: low-intensity pulsed ultrasound, gelatin hydrogel, BMP, bone formation

JOURNAL OPEN ACCESS

2024 Volume 22 Issue 2 Pages 53-59

DOI https://doi.org/10.11223/jarde.22.53

Details

Abstract

This study was designed to investigate the effects of low-intensity pulsed ultrasound (LIPUS) on bone formation at subperiosteal sites in Wistar rats, utilizing gelatin hydrogel (Gel) containing bone morphogenetic protein-2 (BMP-2). The experimental sites were divided into four groups: Implantation with Gel containing BMP-2 (BMP group) and Gel only (Gel group), LIPUS treatment was performed every three days after implantation with Gel containing BMP-2 (Li-BMP group) and Gel only (Li-Gel group).

Three weeks after implantation, new bone formation was observed in all groups. The thickness of new bone (TNB) in the Li-BMP and BMP groups was significantly greater than that in the Li-Gel and Gel groups (p < 0.05). The TNB in the Li-Gel group was significantly greater than that in the Gel group (p < 0.05). The newly formed bone in the Li-BMP group was nearly integrated with the original bone.

These results suggest LIPUS enhances bone formation and accelerates bone integration when used with gelatin hydrogel containing BMP-2.

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