Effects of Four Types of Hydroxyapatite-containing Dentifrices and Coral-derived Hydroxyapatite Powder on Cell Viability

Abstract

We performed this experiment using 4 kinds of dentifrice containing hydroxyapatite as a component and coral-derived hydroxyapatite. These are insoluble in water, and cell viability was investigated using the three-dimensional culture method with collagen in addition to the two-dimensional culture method. As a result, the cell viability of three paste-type dentifrices was extremely low, but that of one powder-type dentifrice was relatively high. In addition, the cell viability of coral-derived hydroxyapatite was similar to that in the control group. However, strongly cytotoxic products may markedly influence bacteria; they may be appropriate for use in the oral cavity where many microorganisms exist. The cell viability of the eggshell-derived powder-type dentifrice was higher than the values of the paste-type products, and this dentifrice should be selected in accordance with purpose. In addition, a coral-derived product adsorbs bacteria, pigments, odor, lipids, glucose, and protein easily, and may be applied to dentifrices in the future.