Effect of Zinc Oxide on Cell Viability in Acidic or Alkaline Extracellular Environments

抄録

In vitro studies using cultured cells in dental biomaterial research have predominantly been conducted under near-neutral culture conditions, while investigations under acidic or alkaline conditions have been limited. To date, few studies have addressed the fundamental question of how fluctuations in extracellular pH affect cell viability as basic in vitro data. In the present study, we examined changes in cell viability in response to zinc oxide under acidic and alkaline extracellular pH conditions using the MTT assay.

The effect of zinc oxide concentrations on cell viability was smoller under alkaline conditions than under acidic conditions, when compared with the control. Under alkaline conditions, cell viability increased and the IC50 value was higher than in the control. Future research to investigate not only zinc oxide, but also other metal ions and inorganic or organic materials is needed. These studies may provide fundamental data on dental biomaterials in the biological environment and potentially serve as important sources of information in the field of regenerative medicine.