Journal of Oral Tissue Engineering
Online ISSN : 1880-0823
Print ISSN : 1348-9623
ISSN-L : 1348-9623
Original Article
Influence of the Human Skin Model on Cell Viability Test and the Formation Test of Tubule-like Structures by Very Low Concentrations Copper, Titanium and Zinc Ions
Author information

2008 Volume 6 Issue 1 Pages 56-61


The positive dissolution of the trace element ion from the alloy for prosthetic dentistry is expected in Regenerative Dentistry. Vascularization, which carries oxygen and nutrition, is indispensable to tissue generation. We investigated the influence of the human skin model on cell viability test and the formation test of tubule-like structures is very low concentration copper, titanium and zinc ions. In both experiments, the influences of metal ions significantly differed. The cell viability test using a human skin model showed significant differences in the reaction to slight cell stimulate on. In testing the formation of tubule-like structures, the influence of the singularity of HUVEC should be considered. In the dentistry field especially, the dissolution of chemical substances from material, applications in reparative dentistry from prosthetic dentistry material to saliva is expected. The development of technology to control the dissolution of metal ions is also required. Basic experiments focusing on specific metal ions or changes in the exposure period need to be accumulated in future studies.

Information related to the author
© Japanese Association of Regenerative Dentistry
Previous article Next article