Pre-intercalated DNA/Lipid/PLGA Film as a Drug Carrier

Abstract

Complexes of daunorubicin hydrochloride (DH)-intercalated DNA and artificial lipids were synthesized. These DH-pre-intercalated DNA/lipid complexes were blended with PLGA (poly (D, L-lactide-co-glycolide)) to prepare DH-releasable films as a carrier material for a drug delivery system (DDS). The films could release DH in PBS solution. The amount of DH released was dependent on the chemical structure of the lipid and initial concentration of DH in the pre-intercalated DNA. Pre-intercalation slowed the release of DH better than post-intercalation. The DH released caused a reduction in cell viability during the culture of L-929 mouse fibroblasts. DH-pre-intercalated DNA/lipid/PLGA films have potential for the controlled-release of DH.