2011 年 8 巻 3 号 p. 173-180
Bone morphogenetic protein-2 (BMP-2) may play a major role in osteoinduction and osteoclast differentiation. The influence of BMP-2 conditioning in graded doses to root dentin surface on periodontal wound healing was examined.
Twenty-four periodontal defects were surgically created on the buccal roots of four beagle dogs. The denuded root dentin surfaces were demineralized with 24% EDTA, 0.1 and 1.0 μg/μL BMP-2 solution (15 μL) was applied, and the groups were labeled as low-dose BMP (BL) and high-dose BMP (BH), respectively. In the control roots, phosphate-buffered saline was applied to the root surface. Specimens were histologically analyzed 16 weeks after surgery.
Periodontal wound healing was stimulated by conditioning with BMP-2. Formation of alveolar bone and cementum-like tissue was observed; however, in BH group, ankylosis and root resorption were accelerated in the defect area, and there was little evidence of periodontal ligament formation between alveolar bone and root surface.
