Development of Method for Early Diagnosis of Mastitis in Dairy Cows by Chemiluminescence

Hideyuki TAKAHASHI; Masaharu ODAI; Kenji MITANI; Shozo ARAI; Nobuo KOIZUMI; Shigeki INUMARU; Yuichi YOKOMIZO

doi:10.6090/jarq.35.131

Animal Husbandry

Development of Method for Early Diagnosis of Mastitis in Dairy Cows by Chemiluminescence

Hideyuki TAKAHASHI, Masaharu ODAI, Kenji MITANI, Shozo ARAI, Nobuo KOIZUMI, Shigeki INUMARU, Yuichi YOKOMIZO

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Keywords: phagocytic leukocytes, milk chemiluminescence, GM-CSF, Staphylococcus aureus, mastitis diagnosing apparatus

JOURNAL FREE ACCESS

2001 Volume 35 Issue 2 Pages 131-136

DOI https://doi.org/10.6090/jarq.35.131

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Abstract

The chemiluminescence (CL) assay measures the ability of a phagocyte to produce light. Milk CL assay can detect an early stage of mastitis precisely because phagocytic leukocytes, such as neutrophils, accumulate in milk as a protective initial reaction after bacterial invasion. Based on the emission of CL by phagocytic leukocytes in milk, we developed a method to diagnose mastitis at an early stage.

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