Japan Agricultural Research Quarterly: JARQ
Online ISSN : 2185-8896
Print ISSN : 0021-3551
ISSN-L : 0021-3551
Biotechnology
Effects of Complex Organic Extracts on Plantlet Regeneration from PLBs and Plantlet Growth in the Doritaenopsis Orchid
Abu Reza Md. Mahfuzur RAHMANM. Obaidul ISLAMA.K.M. Azad-ud-doula PRODHANSyoichi ICHIHASHI
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2004 Volume 38 Issue 1 Pages 55-59

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Abstract

Potato extract (PE), corn extract (CE) and papaya extract (PAE) significantly enhanced plantlet regeneration from PLBs (protocorm-like bodies) when 50 mL L-1 of an extract was supplemented to the New Phalaenopsis (NP) medium containing sucrose. Among the treatments, CE 50 mL L-1 induced the highest rate (66.63%) of plantlet regeneration from PLBs. Organic extracts of potato, corn and papaya at 50-100 mL L-1 in NP medium also enhanced the subsequent growth of mini-plantlets. However, a higher level (200 mL L-1) of extract in the medium inhibited the growth of the plantlets. Among these extracts, PE at 100 mL L-1 showed the optimum effect on the acceleration of shoot growth while CE at the same concentration was optimum for root growth.

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© 2004 Japan International Research Center for Agricultural Sciences
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