Japan Agricultural Research Quarterly: JARQ
Online ISSN : 2185-8896
Print ISSN : 0021-3551
ISSN-L : 0021-3551
Biotechnology
Application of RLGS Method for Detection of Alteration in Tissue Cultured Plants
Tomoko TAKAMIYAYuhko OHTAKESaeko HOSOBUCHITomotsugu NOGUCHIMakoto KAWASEYasufumi MURAKAMIHisato OKUIZUMI
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Keywords: DNA methylation
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2008 Volume 42 Issue 3 Pages 151-155

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Abstract

Tissue culture is used as a tool to propagate seedlings massively in industry. However, unfavorable genomic alteration that affects phenotypic characters occurs in the procedure. A technique which scans these genetic changes genome-wide can enables us to discriminate the clone that has genomic alteration at early stages. In this study, we used restriction landmark genome scanning (RLGS) to detect alterations among ramets of rice, which redifferentiated from a callus that had been cultured for 4 weeks. As a result, 6% (10 spots) of the total (161 spots examined) were different between two ramets. One spot out of ten was cloned and was located on a predicted gene. Application of a PCR-based method to the RLGS spot region detected one methylation alteration among the ramets. Accordingly, RLGS was successful in surveying methylation alteration events during tissue culture in plants and it should be possible to use this method for monitoring of alteration levels.

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© 2008 Japan International Research Center for Agricultural Sciences
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