Measurement of DNA length on video of fluorescence microscope by pix2pix trained by molecular dynamics simulation

Abstract

For the automatic measurement of DNA length in the video captured by fluorescence microscope, image processing method using pix2pix is developed. To increase the accuracy of the measurement in low-resolution video, a method of inputting multiple time-series frames to pix2pix are proposed. To generate the training data, molecular dynamics simulation is employed to prepare dummy video. The trained pix2pix by multiple frames input can generate more accurate images in a scene of DNA breakage than the pix2pix trained by single frame input.