Abstract
Automated analysis of methamphetamine (MA) and amphetamine (AP) in human urine was made by using PrepStation® with gas chromatograph / mass spectrometer (GC / MS). Using “an extraction-derivatization solution”, which was made up of chloroforrn, isopropylalcohol and propylchloroformate (150:50:1, v/v/v), it was possible to extract and derivatize MA and AP simultaneously. This method was applied to actual urine specimens. The analysis time for 1 sample was approximately 30 minutes. Linear calibration curves, using the internal standard method, were obtained throughout the concentration range from 0.25 to 5 μg/ml in either the scan or selected ion monitoring (SIM) mode. Using a scan mode the detection limits were 1 μg/ml for both MA and AP and using a SIM mode the limits were 0.25 μg/ml for both MA and AP. The system studied here appeared simple and useful towards its application in daily duties.
