Abstract
It has been known that exfoliation of endothelial cell is a factor of atherogenesis. Already, we reported that we observed the circulating exfoliated endothelial cells in man by Hladovec's method with Giemsa staining and Ruthenium Red Staining.
In the present study, we examined these cells with direct immunofluorescent staining using anti-human factor VIII serum.
This study was performed on 14 healthy men, 8 healthy women and 5 hemodialysis patients.
The blood was collected from extracorporal artificial vessel in hemodialysis patients.
Results obtained were as follows;
(1) The average numbers of the circulating endothelial cells were 2.77 cells/9μl PRP in men, 2.74 cells/9μl PRP in women, and 1.8 cells/9μl PRP in hemodialysis patients respectively.
(2) The diameter of these cells is about 20-50μm in average.
(3) The circulating endothelial cells showed, as a rule, polygonal shape, often with folded or rolled suggesting a marked flatness in Giemsa staining.
(4) The membrane of the cell was stained with Ruthenium Red.
(5) In the flourescent staining, we could observe the intense fluorescence in the cell with antihuman factor VIII serum.
These results were confirmed that the endothelial cells existed in the blood stream.
