培養心筋細胞の拍動の観察

抄録

Myocardial cells were isolated from the mouse embryo and cultured in microplate. The effects of the Ca²⁺-antagonist (nifedipine, diltiazem) on frequency of spontaneous beating of the myocardial cells were studied and it was found that noradrenaline-stimulated beating was inhibited reversibly, by adding nifedipine (100ng/ml). Diltiazem (active form) showed an inhibitory effect at 1μg/ml on noradrenaline-stimulated beating while 10 times higher concentration of diltiazem of inactive form was required to exert a similar inhibitory effect. Nicomol and cadmium didd not affect the beating at 0.1μg/ml and 10μg/ml, respectively.