Abstract
Apoprotein A-1- and B-100-containing particles were isolated by selective affinity columns prepared with monoclonal antibodies. The effects of probucol and clinofibrate on cholesterol and protein concentrations in these particles were studied in non insulin dependent diabetic patients. Total plasma cholesterol levels in the 2 groups were both reduced significantly after 8 weeks of treatment, but during this period, fasting blood glucose and hemoglobin HbA-1 levels were almost unchanged in the 2 groups. Cholesterol and protein contents in apoprotein A-1 particles after treatment with probucol were both reduced, but not significantly. Cholesterol and protein contents in apoprotein A-1 particles after treatment with clinofibrate were both significantly increased (p<0.05). Cholesterol contents in apoprotein B-100 particles after treatment with probucol or clinofibrate were both reduced (p<0.05) significantly. Cholesterol contents in other lipoprotein fractions after treatment with probucol or clinofibrate were both reduced (p<0.05) significantly. Protein contents in apoprotein B-100 particles were significantly increased (p<0.05) after treatment with probucol, and were reduced after treatment with clinofibrate, but not significantly. The ratio of esterified cholesterol/free cholesterol in apoprotein A-1 or B-100 were almost unchanged with probucol or clinofibrate, but the ratio of total cholesterol/protein in apoprotein B-100 was reduced (p<0.05) with probucol and the ratio in apoprotein A-1 was increased (p<0.05) with clinofibrate. It may be important for preventive treatment of atherosclerosis to analyze apoprotein A-1 and B-100 particles.
