抄録
We examined the characteristics of lipoproteins in PAN (puromycin aminonucleoside)-induced nephrotic rats. The TRL (triglyceride-rich lipoproteins), IDL, LDL and HDL were all detected in nephrotic rats as prominent peaks by HPLC (high-performance liquid chromatography), while only HDL was dominant in the control rats. The retention time of each fraction in the nephrotic rats was short compared to that in the controls, indicating that the particle size of the lipoproteins became larger. The total lipoprotein concentration in the nephrotic rats was 9.4-fold higher than that of the controls and particularly, the increase in LDL was greatest. The chemical composition of each lipoprotein isolated by sequential ultracentrifugation was also altered in the nephrotic rats. The percentage of protein was significantly decreased in all fractions suggesting that the lipoprotein density is reduced.
Serum apo A-I and B was increased 8.5- and 13-fold, respectively, but apo A-IV was reduced by 56% in the nephrotic rats. The SDS-gel patterns showed abundant apo BL in the TRL of the nephrotic rats, whereas apo BH was dominant in the TRL of the control rats. Additionally, nephrotic HDL2 contained markedly increased apo A-I and significantly decreased apo A-IV and apo E compared to the control HDLL.
