抄録
The Oxidative modification of low-density lipoprotein (LDL) could contribute to atherosclerosis as a result of its cytotoxic effect, uptake by the scavenger receptor, and its influence on monocyte and macrophage migration. Ascorbate is an important watersoluble, chain-breaking antioxidant in humans.
In this study, we examined the effect of ascorbate on the Cu2+-induced oxidative modification of LDL. LDL was incubated for 24 hours with 2.5μM copper (Cu2+) in phosphate-buffered saline (PBS) in both the presence and absence of ascorbic acid (20μg/ml, 25μg/ml, 30μg/ml, and 35μg/ml). Ascorbate significantly inhibited the oxidative modification of LDL, as indicated by both the decreased electrophoretic mobility and the linoleic acid content. Oxidative modification was prevented in a concentration-dependent manner by the addition of ascorbate.
Our data suggests that ascorbate may play an important physiological role in protection against the oxidative modification of LDL.
