Abstract
It is well known that heparin and heparinoids (HPS) play an important role in the activation of lipoprotein lipase (LPL), whereas it is not so clear whether HPS are necessary any more for the activated LPL itself on the action in the organs and/or tissue or not. On this paper, we tried to detect the relationship between heparin, heparinase and activated LPL in vitro. Citrated post-heparin plasma (PHP) from rabbit was used in this experiment. Heparin content and LPL activity (by NEFA) were detected with and/or without heparinase. Inhibitory effect of protamine sulfate on LPL activity was also detected.
(Results)
(1) Heparin content was markedly decreased by adding of heparinase in vitro.
(2) Heparin content of PHP was abruptly decreased on addition of heparinase, whereas no inhibitory changes of LPL activity were observed on this PHP.
(3) Dose-responded inhibition by protamine sulfate on LPL activity was observed in lower concentration, while in higher concentration the inhibitory effect reached in plateau.
From results mentioned above, we supposed that LPL activity in PHP was mostly induced from peripheral organs, and that heparin was no more necessary for the activated LPL.
