Preparation of gelatin scaffold and fibroblast cell culture

Abstract

A gelatin gel sheet was UV-irradiated to raise the gel melting temperature T_m. Three second of T_m increased proportionally to irradiation time t_UV. The sheet of which T_m was larger than the culture temperature 37°C was used as a cell culture scaffold. Human fibroblast cells WI-38 adhered to the sheet and dug deeply into it regardless of t_UV, while cancer cells WI-38-VA-13 formed spheroids and did not adhere to the sheet individually at high UV dose. The cell selectivity of the scaffold could be used for biological and medical studies.