Simultaneous Determination of Ethyl alcohol, Polyols, and Sugars in Grape Must and Wine by High-Performance Liquid Chromatography.

Abstract

A new highly efficient determination method of ethyl alcohol, polyols, and sugars in grape musts and wines was established by a high-performance liquid chromatography with a strong cation-exchangeresin, calcium form, and a refractive index detector. Ethyl alcohol, glycerol, sorbitol, glucose, fructose, and sucrose were satisfactorily separated on Diaion CK-08 S (Ca²⁺) with water as the eluent within about 30 min. The relationship between peak area and concentration of the above mentioned components was linear. Recovery of the components found in the wine was 91 to 101%. The results determined by the method were excellently reproducible. This procedure was successfully applied to various grape musts and wines (16 samples). A significant amount of glucose, fructose, and glycerol was detected in the German noble wines.