Abstract
Nitrate reducing bacteria had been reported to play an important roll in yamahai-moto, a seed mash of traditional method for sake brewing, but in some cases these bacteria do not grow in yamahai-moto.Therefore, a method to utilize freezedried cells of nitrate reducing bacteria for yamahai-moto making was investigated.Among tested 13 strains of nitrate reducing bacteria isolated from sake breweries, only Pseudonzonas sp.P 567 renatured well at 7°C from freeze-dried cells.The addition of freeze-dried cells of P 567 caused NO2 formation in yamahai-moto at 7°C in a similar manner to the addition of its culture broth.
