Angiotensin I-converting enzyme inhibitory factors present water extracts of Sakebushi

Abstract

Sakebushi is made from dried protease-treated chum salmon (Oncorhynchus keta) that have released their spawn.
We carried out in vitro testing of water extracts of Sakebushi for angiotensin I-converting enzyme (ACE) inhibitory peptides. Water extracts (2%(w/v) dried fish) contained 0.10% total nitrogen, 6.10±0.62 mg/ml total protein and 2.53±0.30 mg/ml free amino acids, and demonstrated 58.4% inhibition against ACE, and IC₅₀, values of 1.148 mg/ml.