An animal model of Parkinson's disease produced by rotenone intraperitoneal injection

抄録

Objective Modeling a stable and reproducible animal model of Parkinson's disease by rotenone intraperitoneal injection for further pathogenesis study of PD. Methods Twenty-seven male Wistar rats were used, which were randomly divided into three groups on average (Rotenone Injection, Vehicle Injection and Normal group). Rats of rotenone injection group (RIG) were administered rotenone (3.0 mg/kg/day) in a specialized vehicle through daily intraperitoneal injection; rats of vehicle injection group (VIG) were administered only special vehicle in the same way, rats of normal group (NG) didn't receive any injection. Checked the changes of its behavior and numbers of SNpc neurons to determine the model successful or not. Results Six rats of RIG developed part of Parkinson's symptoms at different time. All rats of RIG emerged behavioral deficits through rearing behavior testing and square bridge testing, and the mean number of SNpc neurons showed significant reduction. There were no behavioral deficits and changes of the mean number of SNpc neurons in rats of NG and VIG. Conclusion Model of Parkinson's disease by rotenone intraperitoneal injection was successfully established. This form of the rotenone model is stable and easy to reproduce, and may provide a new excellent supporter to related studies.