JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
Online ISSN : 1881-1299
Print ISSN : 0021-9592
Growth Characteristics of Plant Hairy Roots and Their Cultures in Bioreactors
Masahito TayaAkihiko YoyamaOsamu KondoTakeshi KobayashiChiaki Matsui
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1989 Volume 22 Issue 1 Pages 84-89

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Abstract
Plant hairy roots from horseradish, carrots, etc. transformed by the soil bacterium Agrobacterium rhizogenes showed active growth in the phytohormone-free Murashige–Skoog medium with sucrose as a carbon source. Reticulate polyurethane foam was an appropriate support for the immobilization of hairy root cells. The hairy roots of 11 kg-dry cells·m–3 (110 kg-fresh cells·m–3) were obtained in a 31-d culture in an air-lift column bioreactor with immobilized horseradish cells. A linear relationship was observed between the dry cell mass grown and medium conductivity decrease for hairy root cultures. Thus, it was possible to monitor cell mass concentration during the hairy root culture by on-line measurement of conductivity in the bioreactor system.
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© 1989 The Society of Chemical Engineers, Japan
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