Abstract
In viable animal cells, fluorescein diacetate is hydrolyzed to fluorescein (a fluorescent compound) which is retained in the cells. Fluorescein was effectively extracted from the cells by treatment with hypotonic phosphate buffer (1 mmol·dm–3, pH 7.4) and the extract showed a fluorescent peak at excitation and emission wavelengths of 496.5 and 515 nm, respectively. A linear relationship was confirmed between the viable cell concentrations based on fluorometry and microscopy. Animal cell growth could be successfully monitored based on fluorometry for suspension and microcarrier cultures of animal cells.
