Micro-Plate Based Monolithic Ion-Exchange Chromatography for High Throughput Protein Purification Process Design

Noriko Yoshimoto; Yuko Nishijima; Parvin Akbarzadehlaleh; Sachie Fujii; Mitsuyo Abe; Shuichi Yamamoto

doi:10.1252/jcej.07WE184

Biochemical, Food and Medical Engineering

Micro-Plate Based Monolithic Ion-Exchange Chromatography for High Throughput Protein Purification Process Design

Noriko Yoshimoto, Yuko Nishijima, Parvin Akbarzadehlaleh, Sachie Fujii, Mitsuyo Abe, Shuichi Yamamoto

著者情報

キーワード: High Throughput System, Monolithic Chromatography, Ion-Exchange Chromatography, 96-Well Micro-Plate, Linear Gradient Elution

ジャーナル認証あり

2008 年 41 巻 3 号 p. 200-205

DOI https://doi.org/10.1252/jcej.07WE184

詳細

抄録

As a method for surveying efficient separation conditions for the mobile phase, a 96-well micro-plate based monolithic ion-exchange chromatography system was developed. Discontinuous flow was accomplished by a vacuum manifold. Linear salt gradient elution experiments were carried out with this system and the elution curves were compared with those for monolithic disk column IEC. The normalized gradient slope–the peak salt concentration data for the micro-plate system agreed with those for the monolithic disk column IEC. This system is useful as the liquid volume needed for the experiment is highly reduced, and large amounts of data are obtained quickly.

責任著者(Corresponding author)

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