Proposing an Ultrasonic Transducer Exciting In-plane Vibration on a Glass Culture Surface

Abstract

It has been reported that ultrasound can be used for bioengineering research such as cell detachment without enzymes resulting in high cell activity. However, the required forces on cells in such research have not been uncovered since ultrasonic waves generated by out-of-plane vibration on the culture surface develop a complex sound field owing to the reflections of the ultrasonic waves in the culture chamber. Therefore, we developed an ultrasonic device that generates in-plane vibration on the culture surface, which is highly attenuated in the culture chamber to avoid reflections. As a result, it was confirmed that there is an area where in-plane vibration is dominantly excited on the glass culture surface with a driving frequency of 580.3 kHz.