最近の研究から
機能性抗体を用いたアデノシンA2A受容体の結晶構造解析
2013 年 55 巻 2 号 p. 103-109
詳細
2013 年 55 巻 2 号 p. 103-109
G protein coupled receptors (GPCR) are involved in a wide variety of physiological functions, and thus are the targets for most of today's medicine. Here we report the raising of a monoclonalantibody against human adenosine A2A receptor that works as strong inverse agonist, and describe the crystal structure of the receptor in complex with the antibody fragment. The structure reveals that the antibody specifically recognizes the intracellular surface of thereceptor in an inactivated state so as to inhibit the structural changes to the active conformation. Our results suggest a new strategy to modulate the activity of GPCR.
