ミニ特集 精密構造解析
生命科学の飛躍のために一層深まるタンパク質結晶学の役割～タンパク質の超高分解能電荷密度解析
2020 年 62 巻 2 号 p. 106-111
詳細
2020 年 62 巻 2 号 p. 106-111
The electron density distribution of each atom reflects chemical properties of the atom in the molecule. The charge-density analysis at ultra-high resolution can analyze details of the electronic distribution even in protein molecules. In this paper, we introduce a recent our result for the charge density analysis of green fluorescent protein (GFP) at an ultra-high resolution of 0.78 Å. The precise electron density distribution gives information about CH-π and lone pair-π interactions and CH…O/N type non-conventional hydrogen bonds as well as conventional hydrogen bonds. The information about such weak but substantial interactions between the chromophore and the protein environment explains the properties of GFP.
