総合報告
タンパク質結晶化に応用可能な新規小型抗体フォーマットFv-claspの開発
2021 年 63 巻 2 号 p. 113-120
詳細
2021 年 63 巻 2 号 p. 113-120
In the protein crystallography, antibodies are frequently used as ‘crystallization chaperones’, where their binding facilitates production of high-quality diffracting crystals of complex macromolecules that are otherwise resistant to crystallization. To develop an ideal antibody fragment, we have designed a novel antibody fragment format, called ‘Fv-clasp’, that is a fusion of an anti-parallel coiled-coil structure derived from the hMst1 SARAH domain to the Fv fragment of an antibody. We have demonstrated that Fv-clasp has superior properties over conventional antibody fragments including Fab and single-chain Fv（scFv）in terms of producibility, stability, and crystallizability.
