SPring-8タンパク質結晶解析ビームラインにおける高効率データ収集システムの利用技術の開発

抄録

Automated data collection by the ZOO system has realized effective data collection for protein structure determination at SPring-8. Using the ZOO system, it is now available to collect diffraction data from hundreds of single crystals within a day. In order to analyze crystal structures using the huge amount of data, we have been developing an automated structure analysis pipeline. Furthermore, we have applied the highly efficient data collection systems of SPring-8 to high-throughput ligand screening using protein crystals.

Here we report our recent developments on the automated structure analysis pipeline NABE system and the ligand screening pipeline using an acoustic liquid handler ECHO.