Humid Air and Glue-coating method（HAG法）を用いた生理学的環境での銅含有アミン酸化酵素の構造解析

抄録

It is difficult to perform the thermodynamic analysis of conformational changes occurring in protein crystals by conventional cryogenic X-ray crystallography because flash-cooled crystals have ambiguous information about temperature. We have developed a humid air and glue-coating（HAG）method in which the crystals are coated with a water-soluble polymer to control humidity. This method realized X-ray crystallographic analysis with controlled temperature and humidity in a non-cryogenic environment. In this paper, we report details of the HAG method and the thermodynamic analysis of conformational changes of copper-containing amine oxidase using the HAG method.