放射光を用いた室温シリアル結晶構造解析

抄録

Cryo-crystallography is usually used at synchrotron macromolecular crystallography（MX）beamlines in order to mitigate radiation damage. However, the structures determined at cryogenic temperatures sometimes hide conformational states observed at room temperature. Serial femtosecond crystallography（SFX）developed at XFELs demonstrated that room temperature structure analysis is possible even using micro-crystals. Following these successes, serial crystallography is also performed at synchrotron MX beamlines aiming at room temperature structure determination and time resolved structure analysis. In this review, synchrotron serial crystallography（SSX）performed at synchrotron MX beamlines in worldwide is summarized, and development of SSX at SPring-8 MX beamlines is introduced together with ongoing project toward time resolved experiments.